PCR ile tanı konan COVID-19 vakalarında antikor yanıtlarının değerlendirilmesi

Özet

Molecular diagnosis is essential in the diagnosis of COVID-19. The presence of antibodies such as IgG and IgM in serological samples indicates that the person has been exposed to the virus. In this study, it was aimed to determine the post-vaccine antibody responses and its change over time in patients diagnosed with COVID-19 by PCR method. Adult cases diagnosed with COVID-19 by Real Time PCR (Bio-Speedy COVID-19 RT qPCR Bioeksen, Istanbul) between May 2020 and April 2022 were included in the study. The data of these cases were reviewed retrospectively. The presence of COVID-19 IgG and IgM antibodies was investigated by immunochromatographic method (Meril Diagnostics, India). A total of 56 cases aged between 23 and 52 were included in the study. IgG positivity was found in 49 cases (87.5%), and IgM positivity was found in two (3.6%) cases. While seven of the total 56 cases did not receive any vaccination; 1 of the remaining 49 cases received one dose, 21 cases two doses, 23 cases three doses, and 4 cases four doses of COVID-19 vaccine. Six (85.7%) of the seven patients who did not receive the vaccine were found to be IgG positive. IgG positive in 43 (87.8%) and IgM positive in one (2.0%) of 49 cases who had at least one dose of vaccination. IgG was found to be negative in three (14.3%) of 21 cases who received two doses of vaccination, two (8.7%) of 23 cases who received three doses of vaccination, and one of four cases who had four doses of vaccination. It was determined that although all patients had positive SARS-CoV-2 PCR test and received one or more vaccine doses, the expected antibody responses might not occur. Due to PCR positivity in the middle of vaccine doses, in some people; vaccines cannot protect patients from SARS-CoV-2.